Journal: NPJ vaccines

Article Title: Zika purified inactivated virus (ZPIV) vaccine reduced vertical transmission in pregnant immunocompetent mice.

doi: 10.1038/s41541-024-00823-1

Figure Lengend Snippet: Fig. 3 | Prevention of brain pathology by ZPIV vaccination in hSTAT2KI mice. Representative images of the fetal brain from the mock-control (a, d), alum (b, e), or ZPIV (c, f) groups at 7 dpi. In the alum group, necrotic bodies (red circle) are scattered throughout the subventricular zone (SV), evident with loss of cellular density (b), which are absent in the fetal brain from the ZPIV group (c) comparable with those from the mock group (a). Coinciding with the brain pathology, ZIKV ENV proteins (blue arrow) were present in the sub- ventricular zone in the alum group (e), but not in the ZPIV group (f), similar with the mock group (d). CP, choroid plexus, fRBC, fetal red blood cells, (arrow- head); SV, subventricular zone. Scale bars = 50 μm.

Article Snippet: Coupling of microsphere beads with the ENV protein of ZIKV The His-tagged envelope protein of ZIKV was purchased from Sino Biological (Wayne, PA) and coupled toMagPlex beads (Luminex Corp) following the protocol previously described38 withminormodifications.

Techniques: Control

Journal: iScience

Article Title: 5-Hydroxymethyltubercidin exhibits potent antiviral activity against flaviviruses and coronaviruses, including SARS-CoV-2

doi: 10.1016/j.isci.2021.103120

Figure Lengend Snippet:

Article Snippet: Recombinant ZIKV NS5 protein , Sino Biological , Cat# 40546-V08B.

Techniques: Virus, Recombinant, Software, Real-time Polymerase Chain Reaction, Fluorescence, Microscopy

Journal: iScience

Article Title: 5-Hydroxymethyltubercidin exhibits potent antiviral activity against flaviviruses and coronaviruses, including SARS-CoV-2

doi: 10.1016/j.isci.2021.103120

Figure Lengend Snippet: EC 50 Values of HUP1108 (HMTU) against flaviviruses

Article Snippet: Recombinant ZIKV NS5 protein , Sino Biological , Cat# 40546-V08B.

Techniques:

Journal: iScience

Article Title: 5-Hydroxymethyltubercidin exhibits potent antiviral activity against flaviviruses and coronaviruses, including SARS-CoV-2

doi: 10.1016/j.isci.2021.103120

Figure Lengend Snippet: HMTU 5′-triphosphate impairs RNA extension by viral RdRp (A) Analysis of chain termination activity of HMTU 5′-triphosphate (HMTU-TP) by the primer extension assay catalyzed by ZIKV RdRp. The RNA P/T used in this assay is indicated at the top (small black circles indicate the incorporation sites of ATP). ZIKV NS5 and RNA P/T were incubated with ATP analogs (500 μM ATP, 100 μM 3′-dATP, or serially diluted HMTU-TP), as indicated in each lane. Primer extension reactions were initiated by the addition of ATP, GTP, CTP, and UTP (100 μM each). The reactions were performed at 30°C for 2 h and stopped by the addition of quenching buffer. The products were resolved by denaturing PAGE. (B) Relative band intensities of fluorescently labeled RNA primers. Relative fluorescence intensities of each RNA primer (white arrowhead in Figure 5 A) were normalized by the RNA sample without ATP or HMTU-TP (black bar, RNA only).

Article Snippet: Recombinant ZIKV NS5 protein , Sino Biological , Cat# 40546-V08B.

Techniques: Activity Assay, Primer Extension Assay, Incubation, Labeling, Fluorescence

Journal: iScience

Article Title: 5-Hydroxymethyltubercidin exhibits potent antiviral activity against flaviviruses and coronaviruses, including SARS-CoV-2

doi: 10.1016/j.isci.2021.103120

Figure Lengend Snippet:

Article Snippet: Recombinant ZIKV NS5 protein , Sino Biological , Cat# 40546-V08B.

Techniques: Virus, Recombinant, Software, Real-time Polymerase Chain Reaction, Fluorescence, Microscopy